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VueLife® AC

Hematopoietic Stem Cells

o    Title: Genetic modification of human hematopoietic cells: preclinical optimization of oncoretroviral-mediated gene transfer for clinical trials

·   Products Used: VueLife® Bags (For pre-stimulation of CD34+ cells) and VueLife® AC Bags (For transduction)

·   Formal Citation: Budak-Alpdogan, T., & Rivière, I. (2009). Genetic modification of human hematopoietic cells: preclinical optimization of oncoretroviral-mediated gene transfer for clinical trials. Methods in Molecular Biology (Clifton, N.J.), 506, 33–58. http://doi.org/10.1007/978-1-59745-409-4_4

 

T Cells

o    Title: Phenotypic and Functional Attributes of Lentivirus-modified CD19-specific Human CD8+ Central Memory T Cells Manufactured at Clinical Scale

·   Products Used: 32-mL VueLife® AC Bags (For spinoculation and initial T-cell expansion), 730-mL VueLife® Bags (For T-cell expansion)

·   Formal Citation: Wang, X., Naranjo, A., Brown, C. E., Bautista, C., Wong, C. W., Chang, W.-C., … Jensen, M. C. (2013). Phenotypic and functional attributes of lentivirus-modified CD19-specific human CD8+ central memory T cells manufactured at clinical scale. Journal of Immunotherapy (Hagerstown, Md. : 1997), 35(9), 689–701. http://doi.org/10.1097/CJI.0b013e318270dec7

o    Title: Chimeric antigen receptor-redirected CD45RA-negative T cells have potent antileukemia and pathogen memory response without graft-versus-host activity

·   Products Used: VueLife® Bags (For large-scale transduction)

·   Formal Citation: Chan, W. K., Suwannasaen, D., Throm, R. E., Li, Y., Eldridge, P. W., Houston, J., … Leung, W. (2014). Chimeric antigen receptor-redirected CD45RA-negative T cells have potent antileukemia and pathogen memory response without graft-versus-host activity. Leukemia, (April), 1–9. http://doi.org/10.1038/leu.2014.174

 

NK Cells

o    Title: Clinical-Grade Generation of Active NK Cells from Cord Blood Hematopoietic Progenitor Cells for Immunotherapy Using a Closed-System Culture Process

·   Product Used: 290-mL and 750-mL VueLife®  AC Bags (For UCB initial cell expansion and differentiation)

·   Formal Citation: Spanholtz, J., Preijers, F., Tordoir, M., Trilsbeek, C., Paardekooper, J., de Witte, T., … Dolstra, H. (2011). Clinical-grade generation of active NK cells from cord blood hematopoietic progenitor cells for immunotherapy using a closed-system culture process. PLoS ONE, 6(6). http://doi.org/10.1371/journal.pone.0020740

 

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